Journal: Pharmaceutical Biology

Article Title: Jinlida granules ameliorate the high-fat-diet induced liver injury in mice by antagonising hepatocytes pyroptosis

doi: 10.1080/13880209.2022.2029501

Figure Lengend Snippet: JLD ameliorated HFD-induced pyroptosis in mice. (A) qRT-PCR analysis of proptosis-related gene caspase 1, IL-1β and IL-18 expression in liver ( n = 3). (B) Western blot analysis of liver proptosis-related protein NLRP3, pro-caspase 1 and IL-1β expression in liver ( n = 3). Data were normalised to GAPDH for western blot. Data are shown as the mean ± SD. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CON + LFD. * p < 0.05 vs. CON + HFD.

Article Snippet: The membranes were blocked using Odyssey blocking Buffer for 1 h and incubated with the primary antibodies specific for NLRP3 (15101, CST; 19771, Proteintech), caspase-1 (3866, CST; 22915, Proteintech), IL-1β (2105, Abcam), p -IKB-α (133462, Abcam), IKB-α (76429, Abcam) and GAPDH (5174, CST) overnight at 4 °C.

Techniques: Quantitative RT-PCR, Expressing, Western Blot

Journal: Pharmaceutical Biology

Article Title: Jinlida granules ameliorate the high-fat-diet induced liver injury in mice by antagonising hepatocytes pyroptosis

doi: 10.1080/13880209.2022.2029501

Figure Lengend Snippet: JLD protects HepG2 against pyroptosis induced by FFA. Expression of NLRP3, caspase 1, IL-1β, p -IKBα and IKBα protein in HepG2 cells. Data were normalised to GAPDH for western blot. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CON. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. FFA

Article Snippet: The membranes were blocked using Odyssey blocking Buffer for 1 h and incubated with the primary antibodies specific for NLRP3 (15101, CST; 19771, Proteintech), caspase-1 (3866, CST; 22915, Proteintech), IL-1β (2105, Abcam), p -IKB-α (133462, Abcam), IKB-α (76429, Abcam) and GAPDH (5174, CST) overnight at 4 °C.

Techniques: Expressing, Western Blot